Licania arborea in the Germplasm Resources Information Network (GRIN), U.S. Department of Agriculture Agricultural Research Service. Licania is a plant genus in the family Chrysobalanaceae. Mainly due to deforestation, several Licania arborea · Licania caldasiana · Licania chiriquiensis · Licania conferruminata · Licania fasciculata · Licania grandibracteata · Licania. Espesye sa tanom nga bulak ang Licania arborea. Una ning gihulagway ni Berthold Carl Seemann. Ang Licania arborea sakop sa kahenera nga Licania sa .
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Licania arborea fraction bioactive potential assessment in jurkat and cho-k1 cell lines. II Universidad de Antioquia.
Grupo Productos Naturales Marinos. Several species of Chrysobalanaceae plants have shown a variety of biological activities, exhibiting a source of interesting compounds from the pharmacological point of view. To assess the bioactive potential of L. Cytotoxicity of the fractions was assessed via t T rypan blue dye and tetrazolium salt MTT assays.
Genotoxicity was evaluated determining the sister chromatid exchange SCE. Antiproliferative effect was estimated by clonogenic assay and cell cycle progression. Furthermore, proliferative kinetics were assessed by SCE of L. Cytotoxicity of the evaluated fractions led to reduced cell viability and cloning capability. Particularly, fraction F 8 showed genotoxic effect reflected in increments of SCEs frequency, mainly on Jurkat cells.
Cytotoxicity; antiproliferative effect; genotoxicity; cell lines. Valorar el potencial bioactivo de las fracciones de L.
In Venezuela, Brazil, and USA have been found Licania arborea and other arbotea from the Chrysobalanaceae family containing great quantities of substances with fungicide, antitumor, antioxidant, antiviral, antibacterial, and anti-inflammatory effects, such as terpenes. These compounds have been studied in colon, liver, and melanoma tumor cell lines, and Gram positive bacteria and yeast.
Therefore, herein we present the results of preliminary studies of the biological activity of dichloromethanolic fractions of L. The antiproliferative effect by clonogenic assay, cell cycle progression, and proliferative kinetics were tested through sister chromatid exchange SCE. Analysis grade solvents were used in the preparation of extracts and fractions. Silica gel F and silica gel 60 Merck as stationary phase and Ethyl acetate-hexane 3: To determine the secondary metabolic types present in the extract of L.
Each extract was fractioned by fine layer and column chromatography using the conditions previously described.
Licania arborea Images
Afterwards, the plates were shaken for 4 hours, and absorbance at nm was measured in an spectrophotometer Multiskan Spectrum, Thermo Scientific. Each experiment was repeated at least two times and each point was determined in at least six replicates. Viability was calculated using the averages of the experiments through the relationship of arbroea absorbance of the treatments with the corresponding controls. Absolute cloning efficiency ACE was calculated by the relation between the number of observed colonies and the number of plated cells and the relative cloning efficiency RCE by the relation of the treatment ACE with the corresponding ACE control.
Statistical analysis was made by lineal regression using the method reported by Puck and Steffen.
Cells were cultivated in the previously described conditions. The cells were treated during 20 hours and one hour before harvesting the antimitotic agent Colcemid 0.
Later, chromosome slides and differential staining were prepared. To calculate the generation time was used the relation between the exposure time to BrdU and the proliferative cell number PCN according to reported by Wolff and Perry. Phytochemical march allowed qualitative determination of the main groups of chemical constituents present in Licania arborea leaves.
Large amount of amino acids, phenolic compounds, triterpenoids and tannins, moderated presence of flavonoids and leucoantocianidines were detected. However, we found a lack of of nafto- and antroquinones, cardiotonics and alkaloids.
The arborsa content of phenolic compounds may be interesting and indicates a high chance of finding antioxidant activity in the polar extracts from L. Furthermore, IC 50 determination via MTT, antiproliferative effect by clonogenic assay were assessed for the F 10 and F 8 fractions due to the cytotoxic activity and the amount available for further experiments. Besides, genotoxic and antiproliferative effects for xrborea function and proliferative kinetic by SCE were only assessed for F 8 fraction.
All the tested concentrations in both cell lines showed significant differences compared to untreated cells Fig. Figure 3 shows that both cell lines treated with different concentrations of F 8 behave similarly to the corresponding controls.
Slope values close to 0. Therefore, delays or reduction of the cell cycle progression with licannia applied treatments to both cell lines were not evident. SCE results in number and time cycle in both cell lines do not reveal changes in cells treated compared to the control. However, there was increment in the SCEs frequency compared with the control.
Results showed a significant cytotoxic effect of the F 8 and F 10 fractions in Jurkat and CHO-K1 cell lines, being the greater effect observed in the Jurkat cell line. These results are similar to others reports on the chloromethanolic fraction betulinic, pomolic or oleanolic acids and triterpenoids from leaves of Arborda. In contrast, antiproliferative potential determined by cell cycle progression analysis did not cause change in the cell cycle time in both cell lines. Besides, SCE results showed dose dependent differential effect between cell lines.
These data are similar to other reports, in which it is revealed that fractions and extracts of leaves and fruits of Chrysobalanaceae family plants cause genotoxic effect in the topological conformation of plasmids and in bacteria transformation efficiency with previously treated plasmids.
In conclusion, according to the dichloromethanolic fractions reports of L. Further studies are also required to verify bioactivity in other tumor cell lines, as well as to assesss other activities such as, antifungal, antibacterial, and antiviral, liccania others. Plants as a source of anti-cancer agents.
Myricetin glycosides from Licania densiflora. Chemical and biological studies on Licania genus. Studies in Natural Products Chemistry. Flavonoids from Licania apetala and Licania licaniaeflora Chrysobalanaceae.
Evaluation of some Samoan and Peruvian medicinal plants by licanla biosynthesis and rat ear oedema assays. Pentacyclic triterpenes from Chrysobalanaceae species: Anti-herpes simplex virus effect of a seed extract from the tropical plant Licania tomentosa Benth. Phytochemical screening and Extraction: Culture of animal cells: A manual of basic technique.
Nigrosin as a dye for differentiating live and dead ascites cells.
Licania arborea – Wikispecies
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Camargo M, Cervenka J. Pattern of Chromosomal Replication in Synchronized Lymphocytes. Evaluation and Application of Methotrexate Block.
Puck TT, Steffen J. Life cycle analysis of mammalian cells.
Licania arborea – Wikipedia
A method for localizing metabolic events within the life cycle, and its application to the action of colcemide and sublethal doses of x-irradiation. Wolff S, Perry P. Differential giemsa staining of sister chromatids and the study of sister chromatid exchanges without autoradiography. Genotoxic potentiality of aqueous extract prepared licanla Chrysobalanus icaco L.